• Xeno-free hydrogel for organoid formation
• Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources
• Scalable protocol for lab automation
• Easy 20-minute cell harvesting (enzyme-free)

VitroGel^® ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

The VitroGel ORGANOID Discovery Kit (Cat# VHM04-K) includes all four types of organoid hydrogels (VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3, VitroGel ORGANOID-4) which were formulated with various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions. Use the Discovery Kit to screen which organoid hydrogel version works best for your organoid culture.

VitroGel ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The organoid hydrogels can work in conjunction with VitroGel STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.  VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

• Xeno-free, biological functional hydrogel
• Ready-to-use at room temperature (20 min protocol)
• Supports a wide range of cell types and applications
• Easy, efficient, and enzyme-free cell harvesting in 20 min

VitroGel^® Hydrogel Matrix is a ready-to-use, xeno-free (animal origin-free) functional hydrogel for 3D cell culture research. VitroGel Hydrogel Matrix is an optimized formulation of multi-functional ligands and concentrations to support a wide range of cell types for different applications.

“Just Add Cells” – The hydrogel matrix is ready to mix with cell suspension directly. There is no additional adjustment needed.

VitroGel Hydrogel Matrix closely mimics the natural extracellular matrix (ECM) environment to make cells feel more like at home. The hydrogel is room temperature stable, has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. Cells cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.

This user-friendly functional hydrogel creates an excellent balance of simplicity and versatility.

• Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
• High-quality 3D stem cells for differentiation and organoid formation
• Easy scale-up (from laboratory scale to industrial scale)
• Efficient cell harvesting or subculturing

VitroGel^® STEM is a xeno-free (animal origin-free) hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.

This hydrogel system is ready to use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making cell harvesting simple and effective. The 3D stem cell spheroids that are developed using this system can be used for further sub-cultures, patterned differentiations, organoid development, or re-establishing 2D culture morphologies.